報告人：Ciprian Iliescu研究員
Speaker：Dr. Ciprian Iliescu
主持人：Pavel Neuzil教授
Host：Prof.Pavel Neuzil
時間：2018年11月2日，上午10：00-11：00
Time: Nov. 2nd, 2018, 10-11am
地點：航空樓D座機電學院微納系統第一會議室
Location：Youyi Campus, Hangkong Building, School of Mechanical Engineering, No.1 conference room
主題：器官細胞培養芯片和基因療法的微流控技術
Topic：Microfluidics in cell culture and gene therapy
內容簡介：
器官細胞培養芯片是一種近年來興起的微流控芯片技術，在藥物研發中有很大應用前景。本講座著重闡述Iliescu博士團隊在該領域的主要成就：一種三維培養模型，稱為約束球體（CS），它可以在靜態或灌注培養中操作。在該模型中，細胞聚集在球體中，在表面改性的玻璃玻片和微細化的超薄聚對二甲苯C膜之間的三明治構型中穩定化，最大化傳質，并且克服了不均勻的細胞計數和球體大小的問題。 講座將進一步說明微流體動力學流聚焦如何能夠精確控制自組裝納米顆粒，強調DNA壓實。講授兩種方法：第一種方法是基于溶劑質量的快速變化，第二種方法依賴于表面活性劑和DNA溶液通過可調諧寬度的水流的受控擴散混合。這些微流體方法可以成功地用于基因治療應用，以及用于定制定制的軟納米粒子的合成。
Abstract:
The present work underlines the main achievements of Dr. Iliescu in the field: a 3D culture model named constrain spheroids (CS) that can operate in both static or perfusion culture. In this model the cells aggregate in spheroids which are stabilized in a sandwich configuration between surface modified glass slide and a microfabricated ultra-thin Parylene C membrane maximizing mass transfer, and overcome problems with uneven cell count and spheroids size.
Dr. Iliescu will then illustrate how microfluidic hydrodynamic flow focusing enables an accurate control of self-assembling nanoparticles with emphasis on DNA compaction. Two approaches will be illustrated. The first method is based on rapid change of solvent quality and the second one relies on the controlled diffusive mixing of surfactant and DNA solutions through a water stream of tunable width. These microfluidic methods can be successfully used for gene therapy applications as well as for the synthesis of tailored soft nanoparticles.
報告人簡介
Ciprian Iliescu博士，羅馬尼亞人，現任新加坡國立大學“BIGHEART”生物研究中心研究員。于1999年在羅馬尼亞布加勒斯特理工大學獲博士學位，2001年到2003年在新加坡南洋理工大學做博士后。之后在新加坡生物工程和納米技術研究所（IBN）工作十多年里，主要從事基于MEMS技術的organs on a chip, electrical impedance spectroscopy, microfluidics, isolation of the circulating tumor cells, transdermal drug delivery, DNA compaction for gene therapy等交叉學科研究。Ciprian Iliescu博士迄今為止已在著名期刊發表學術論文約158篇，H因子33。
Speaker's Short Biography
Dr. Iliescu got his Ph.D from Polytechnic University of Bucharest in 1999 with a field of microtechnology. He is currently a senior research scientist at BIGHEART institute, National University of Singapore. From 2001 to 2003 he was a postdoctoral fellow at Nanyang Technical University, Singapore. He has worked at Institute of Biotechnology, Singapore for over 10 years. His main research areas are MEMS-microfluidics technology, including organs on a chip, electrical impedance spectroscopy, isolation of the circulating tumor cells, transdermal drug delivery, DNA compaction for gene therapy,interdisciplinary fields, etc. He has published about 158 scientific journal papers and has H-index of 33.
